Hello from our The Binding of Isaac Rebirth Unlock All Characters guide. Are you looking for a quick and easy guide to help you unlock all the characters in The Binding of Isaac Rebirth? You are in the right place, this guide will help you!
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The Binding of Isaac Rebirth Unlock All Characters
Welcome to our The Binding of Isaac Rebirth Unlock All Characters guide. This guide will show you how to unlock all characters in the The Binding of Isaac Rebirth!
Icon Legend
| Image | Name |
 | Afterbirth |
 | Afterbirth + |
 | Rebirth |
 | Red Heart |
 | Soul Heart |
 | Balck Heart |
 | Full Bone Heart |
All Characters
| Image | Name | How To Unlock | Health |
 | Isaac | Unlocked by default | |
 | Magdalene | Have 7 or more Red Heart Containers at one time | |
 | Cain | Hold 55 Coins at one time | |
 | Judas | Defeat Satan | |
 | ??? | Defeat Mom’s Heart 10 times | |
 | Eve | Don’t pick up any Hearts for 2 floors in a row | |
 | Samson | Complete 2 floors in a row without taking damage | |
 | Azazel | Make 3 Deals with the Devil in one run | |
 | Lazarus | Have 4 or more Soul Hearts at one time | |
 | Eden | Complete Chapter 4 | Random |
 | The Lost | Unlocking the lost in Rebirth requires dying to specific things on specific chapters across 4 runs. First, Isaac must die to a Mulliboom in Chapter 1. Magdalene must die to her own bomb in Chapter 2. Judas must die to Mom. Being killed by anything spawned by Mom will not work. Finally, Azazel must die to Satan. | – |
| The Lost | Die in a sacrifice room with missing poster. (Unlock missing poster by defeating The Lamb as Isaac). | – |
 | Lilith | Defeat Ultra Greed as Azazel | |
 | Keeper | Donate 1000 Coins to the Greed Donation Machine |  |
 | Apollyon | Defeat Mega Satan | |
 | The Forgotten | | |
 | Bethany | Defeat Mom’s Heart/It Lives! on hard mode as Lazarus, without dying at all | |
 | Jacob and Esau | Defeat Mother |  |
Tainted Characters
After defeating Mother, a secret door will appear on the 6th floor connected to the north side of the starting room. At some point on a run as whatever character you want to unlock tainted you must put a trinket in a treasure or boss room. Then on floor 6 there will be a dark skull with an “X”. Bomb it for a guaranteed “0 – The Fool” (Telepills if you have Little baggy and ??? if you have little baggy and False PHD). Defeat Mom and take either picture then teleport back. Use the picture to unlock the door and go to the secret area. Then find Dad’s note in the boss and start the ascent. When you get to the floor where you dropped the trinket, find the boss/item room in pick up the Cracked key piece that should be on the ground. When you reach home go to the hallway leading to Mom’s bedroom and there should be a red outline of a door. Walk up to it and use the piece and the tainted unlock will be in the room.
